Sex: Female


  • University of Connecticut, United States of America, Doctor of Philosophy in Chemistry, 1995
  • University of Santo Tomas, España, Manila, Master of Science in Chemistry, 1987
  • University of Santo Tomas, España, Manila, Bachelor of Science in Chemistry, 1980

Field of Specialization:

Chemistry, Spectroscopy, Chemiluminescence, Biotechnology, Toxicology, Medicine, Analytical Chemistry, Physics, Biochemistry, Biosensor Development, Fluorescence Spectroscopy, Sensors, Fluorescent Dye, Glucose


Lam, H., Rao, G., Loureiro, J., & Tolosa, L., (2011) Dual Optical Sensor for Oxygen and Temperature Based on the Combination of Time Domain and Frequency Domain Techniques Talanta Volume 84, No. 1

Lam, H., Kostov, Y., Rao, G., & Tolosa, L., (2008) Low-Cost Optical Lifetime Assisted Ratiometric Glutamine Sensor Based on Glutamine Binding Protein Analytical Biochemistry Volume 383, No. 1

Bartolome, A., Bardliving, C., Rao, G., & Tolosa, L., (2005) Fatty acid sensor for low-cost lifetime-assisted ratiometric sensing using a fluorescent fatty acid binding protein Analytical Biochemistry Volume 345, No. 1

Kostov, Y., Smith, D.S., Tolosa, L., Rao, G., Gryczynski, I., Gryczynski, IZ., Malicka, J., & Lakowicz, J.R., (2005) Directional Surface Plasmon-Coupled Emission from a 3 nm Green Fluorescent Protein Monolayer American Chemical Society and American Institute of Chemical Engineers

Tolosa, L., Ge, X., & Rao, G., (2003) Reagentless optical sensing of glutamine using a dual-emitting glutamine-binding protein Analytical Biochemistry Volume 314, No. 2

Lakowicz, J.R., Gryczynski, I., Piszczek, G., Tolosa, L., Nair, R., Johnson, M.L., & Kazimierz, N., (2000) Microsecond dynamics of biological macromolecules Methods in Enzymology Volume 323

Tolosa, L., Gryczynski, I., Eichhorn, L.R., Dattelbaum, J.D., Castellano, F.N., Rao, G., & Lakowicz, J.R., (1999) Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein Analytical Biochemistry Volume 267, No. 1

Lakowicz, J.R., Gryczynski, I., Gryczynski, Z., Tolosa, L., Dattelbaum, J.D., & Rao, G., (1999) Polarization-Based Sensing with a Self-Referenced Sample Applied Spectroscopy Volume 53, No. 9

Lakowicz, J.R., Castellano, F.N., Dattelbaum, J.D., Tolosa, L., Rao, G., & Gryczynski, I., (1998) Low-Frequency Modulation Sensors Using Nanosecond Fluorophores Analytical Chemistry Volume 70, No. 24

Tolosa, L., Szmacinski, H., Rao, G., & Lakowicz, J.R., (1997) Lifetime-Based Sensing of Glucose Using Energy Transfer with a Long Lifetime Donor Analytical Biochemistry Volume 250, No. 1

Tolosa, L., Malak, H., Raob, G., & Lakowicz, J.R., (1997) Optical assay for glucose based on the luminescnence decay time of the long wavelength dye Cy5TM Sensors and Actuators B Volume 45, No. 2

Kumar, C.V., & Tolosa, L.M., (1994) 2,6-bis(pyren-1-oyl)pyridine: a new fluorescent probe with a high sensitivity to hydrogen-bonding solvents for the development of selective sensors Journal of Photochemistry and Photobiology A Chemistry Volume 78, No. 1

Kumar, C.V., & Tolosa, L.M., (1993) Interaction of Hydrophobic probes with serum albumin - influence of the side chain and exciplex formation at the binding side Journal of Physical Chemistry Volume 97, No. 51

Kumar, C.V., & Tolosa, L.M., (1993) New charge transfer probe for solvent polarity: fluorescent hydrogen-bonding switch Journal of the Chemical Society, Chemical Communications , No. 9

Ge, X., Kostov, Y., Tolosa, L., & Rao, G., (2012) Study on low-cost calibration-free pH sensing with disposable optical sensors Analytica Chimica Acta Volume 734

Tolosa, L., (2009) On the Design of Low-Cost Fluorescent Protein Biosensors Advances in Biochemical Engineering / Biotechnology Volume 116

Lam, H., Kostov, Y., Rao, G., & Tolosa, L., (2009) A luminescence lifetime assisted ratiometric fluorimeter for biological applications Review of Scientific Instruments Volume 80, No. 12

Badugu, R., Kostov, Y., Rao, G., & Tolosa, L., (2008) Development and Application of an Excitation Ratiometric Optical pH Sensor for Bioprocess Monitoring Biotechnology Progress Volume 24, No. 6

Ge, X., Rao, G., & Tolosa, L., (2008) On the Possibility of Real-Time Monitoring of Glucose in Cell Culture by Microdialysis Using a Fluorescent Glucose Binding Protein Sensor Biotechnology Progress Volume 24

Castell, J.V., Tolosa, L., Pinto, S., Donato, M.T., O’Connor, J.E., & Gómez-Lechón, M.J., (2010) Development of multiparametric cell-based protocol to assess and classify hepatotoxicity potential of drugs Toxicology Letters Volume 196

Kumar, C., & Tolosa, L., (1994) New Fluorescent Probe with a High Sensitivity to Hydrogen Bonding Solvents for the Development of Selective Sensors Journal of Photochemistry and PhotoBiology Volume 78

Lakowicz, J.R., Gryczynski, I., Tolosa, L., Dattelbaum, J.D., Castellano, F.N., Li, L., & Rao, G., (1999) Advances in Fluorescence Spectroscopy: Multi-Photon Excitation, Engineered Proteins, Modulation Sensing and Microsecond Rhenium Metal-Ligand Complexes Acta Physica Polonica A Volume 95, No. 1

Tolosa, L., Gryczynski, I., Randers-Eichhorn, L., Dattelbaum, J., Rao, G., & Lakowicz, J., (1999) Fluorescence sensing of glucose Proceedings Volume 3602, Advances in Fluorescence Sensing Technology IV

Lakowicz, J.R., Gryczynski, I., Gryczynski, Z., Dattelbaum, J., Tolosa, L., & Rao, G., (1999) Novel methods for fluorescence sensing Proceedings of SPIE - The International Society for Optical Engineering

Lakowicz, J.R., Gryczynski, I., Gryczynski, Z., Tolosa, L., Randers-Eichhorn, L., & Rao, G., (1999) Polarization-Based Sensing of Glucose Using an Oriented Reference Film Journal of Biomedical Optics Volume 4, No. 4

Kumar, C.V., & Tolosa, L.M., (1999) Exploring Hydrophobic Binding Sites of Calmodulin with Fluorescent Probes: Dynamic Formation of the Excimer Indian Journal of Chemistry - Section B Organic and Medicinal Chemistry Volume 38

Tolosa, L., Kostov, Y., Harms, P., & Rao, G., (2002) Non-invasive Measurement of Dissolved Oxygen in Shake Flasks Biotechnology and Bioengineering Volume 80, No. 5

Kostov, Y., Tolosa, L., O'Connell, K., Anderson, P., Liu, Y., van Beek, N., & Rao, G., (2003) Monitoring of DsRed protein concentration in frozen insect larvae Proceedings Volume 4967, Genetically Engineered and Optical Probes for Biomedical Applications

Murtaza, Z., Tolosa, L., Harms, P., & Lakowicz, J.R., (2002) On the Possibility of Glucose Sensing Using Boronic Acid and a Luminescent Ruthenium Metal-Ligand Complex Journal of Fluorescence Volume 12, No. 2

Tolosa, L., Ge, X., Kostov, Y., Lakowicz, J.R., & Rao, G., (2003) Dual-emitting biosensors for glucose and glutamine from genetically engineered E. coli binding proteins Proceedings Volume 4967, Genetically Engineered and Optical Probes for Biomedical Applications

Ge, X., Tolosa, L., Simpson, J., & Rao, G., (2003) Genetically Engineered Binding Proteins as Biosensors for Fermentation and Cell Culture Biotechnology and Bioengineering Volume 84

Ge, X., Tolosa, L., & Rao, G., (2004) Dual-Labeled Glucose Binding Protein for Ratiometric Measurements of Glucose Analytical Chemistry Volume 76, No. 5

Tolosa, L., & Rao, G., (2006) The Glucose Binding Protein as Glucose Sensor In: Geddes, C.D., Lakowicz, J.R., (eds.) Glucose Sensing. Topics in Fluorescence Spectroscopy, Springer, Boston, MA Volume 11

Bartolome, A., Smalls-Mantey, L., Lin, D.W., Rao, G., & Tolosa, L., (2006) FRET-based glucose monitoring for bioprocessing - art. no. 60980L Proceedings of SPIE - The International Society for Optical Engineering Volume 6098

Ge, X., Lam, H., Modi, S.J., LaCourse, W.R., Rao, G., & Tolosa, L., (2007) Comparing the Performance of the Optical Glucose Assay Based on Glucose Binding Protein with High-Performance Anion-Exchange Chromatography with Pulsed Electrochemical Detection: Efforts to Design a Low-Cost Point-of-Care Glucose Sensor Journal of Diabetes Science and Technology Volume 1, No. 6

Lam, H.T., Kostov, Y., Tolosa, L., Falk, S., & Rao, G., (2012) High Resolution Non-contact Fluorescence Based Temperature Sensor for Neonatal Care Measurement Science and Technology Volume 23, No. 3

Messaoudi, N., Gautier, V., Kthiri, F., Lelandais, G., Mihoub, M., Joseleau-Petit, D., Caldas, T., Landoulsi, A., Bouloc, P., & Richarme, G., (2013) Global Stress Response in a Prokaryotic Model of DJ-1-Associated Parkinsonism Journal of Bacteriology Volume 195, No. 6

Ge, X., Rao, G., Kostov, Y., Kanjananimmanont, S., Viscardi, R., Woo, H.C., & Tolosa, L., (2013) Detection of Trace Glucose on the Surface of a Semipermeable Membrane Using a Fluorescently Labeled Glucose-Binding Protein: A Promising Approach to Noninvasive Glucose Monitoring Journal of Diabetes Science and Technology Volume 7, No. 1

Chatterjee, M., Ge, X., Kostov, Y., Tolosa, L., & Rao, G., (2013) A novel approach toward noninvasive monitoring of transcutaneous COMedical Engineering and Physics Volume 36, No. 1

Woo, H.C., Tolosa, L., El-Metwally, D., & Viscardi, R.M., (2014) Glucose monitoring in neonates: Need for accurate and non-invasive methods Archives of Disease in Childhood - Fetal and Neonatal Edition Volume 99

Kanjananimmanont, S., Ge, X., Mupparapu, K., Rao, G., Potts, R., & Tolosa, L., (2014) Passive Diffusion of Transdermal Glucose: Noninvasive Glucose Sensing Using a Fluorescent Glucose Binding Protein Journal of Diabetes Science and Technology Volume 8, No. 2

Kostov, Y., Ge, X., Rao, G., & Tolosa, L., (2014) Portable system for the detection of micromolar concentrations of glucose Measurement Science and Technology Volume 25, No. 2

Tran, K., Gibson, A., Wong, D., Tilahun, D., Selock, N., Good, T., Ram, G., Tolosa, L., Tolosa, M., Kostov, Y., Woo, H.C., Frizzel, M., Fulda, V., Gopinath, R., Shashidhara Prasad, J., Sudarshan, H., Venkatesan, A., Sashi Kumar, V., Shylaja, N., & Rao, G., (2014) Designing a Low-Cost Multifunctional Infant Incubator Journal of Laboratory Automation Volume 19, No. 3

Chatterjee, M., Ge, X., Kostov, Y., Luu, P., Tolosa, L., Woo, H., Viscardi, R., Falk, S., Potts, R., & Rao, G., (2015) A rate-based transcutaneous CO2 sensor for noninvasive respiration monitoring Physiological Measurement Volume 36, No. 5

Tiangco, C., Brown, S., Sevilla, F., III, Rao, G., & Tolosa, L., (2016) Fluorophore labeled Bioengineered GBP for measurement of transdermal glucose Acta Manilana Volume 64

El-Sayed, M.M.H., Brown, S.R., Mupparapu, K., & Tolosa, L., (2016) The effect of pH on the glucose response of the glucose–galactosebinding protein L255C labeled with Acrylodan International Journal of Biological Macromolecules Volume 86

Tiangco, C., Fon, D., Sardesai, N., Kostov, Y., Sevilla, F., III, Rao, G., & Tolosa, L., (2016) Fiber optic biosensor for transdermal glucose based on the immobilized glucose binding protein Sensors and Actuators B Chemical Volume 242

Peñalber-Johnstone, C., Ge, X., Tran, K., Selock, N., Sardesai, N., Gurramkonda, C., Pilli, M., Tolosa, M., Tolosa, L., Kostov, Y., Frey, D.D., & Rao, G., (2017) Optimizing cell-free protein expression in CHO: Assessing small molecule mass transfer effects in various reactor configurations: Small Molecule Mass Transfer in CHO CFPS Reactors Biotechnology and Bioengineering Volume 114, No. 7

Tiangco, C., Andar, A., Quarterman, J., Ge, X., Sevilla, F., III, Rao, G., Stinchcomb, A., Bunge, A., & Tolosa, L., (2017) Measuring transdermal glucose levels in neonates by passive diffusion: an in vitro porcine skin model Analytical and Bioanalytical Chemistry Volume 409, No. 13

Tran, K., Gurramkonda, C., Cooper, M. A., Pilli, M., Tarris, J., Selock, N., Han, T.-C., Tolosa, M., Zuber, A., Peñalber-Johnstone, C., Dinkins, C., Pezeshk, N., Kostov, Y., Frey, D. D., Tolosa, L., Wood, D., & Rao, G, (2017) Cell?Free Production of a Therapeutic Protein: Expression, Purification, and Characterization of Recombinant Streptokinase Using a CHO Lysate Biotechnology and Bioengineering


  • Principal Investigator, Nanotech Biotechnology Research Project Support Initiative, University System of Maryland and Maryland Department of Business and Economic Development, 2007-2008
  • Balik Scientist Award, Department of Science and Technology, 2008
  • Principal Investigator, Research Project Grant (R01DK072465-01), National Institutes of Health, 2005-2010
  • Principal Investigator, Technologies for Metabolic Monitoring (W81XWH-04-1-0781), U.S. Army Medical Research and Materiel Command, 2004-2007
  • Principal Investigator, Mentored Quantitative Research Career Development Award (K25DK062990), National Institutes of Health, 2003-2006